Mar 20, 2017· This protocol describes a method to extract ribulose1,5bisphosphate carboxylase oxygenase (Rubisco) from diatoms (Bacillariophyta) to determine catalytic performance. This protocol has been adapted from use in cyanobacteria and higher plants (Andrews, 1988; Whitney and Sharwood, 2007). First part (steps A1A3) of the extraction provides a crude extract of Rubisco that is sufficient .